Cell surface proteins are key biomarkers and therapeutic targets, but profiling the surfaceome is challenging. Using Syncell Microscoop® with Synlight-Rich (~350 nm) or Synlight-Pure (~25–50 nm), proteins in microscopy-defined regions are selectively tagged for LC-MS/MS analysis. This enables deep, unbiased mapping of plasma membrane and subcellular proteomes, with Synlight-Pure boosting specificity to ~90%, supporting biomarker discovery and therapeutic target identification in oncology, neurodegeneration, and immunology.
This study highlights Microscoop®’s ability to map the proteomic landscape of primary cilia at unprecedented resolution, identifying 4,233 proteins, including 524 known ciliary proteins critical for assembly, transportation, and signaling.
Microscoop® optoproteomics (combined localization and MS) reveals spatially resolved protein complexes such as spliceosomes and histone complexes, highlighting its potential to advance subcellular biology and uncover intricate cellular interactions.
