Pushing the Limits Unveiling Proteome of Primary Cilia at Unprecedented Resolution Using Microscoop^®

Microscoop® presents a groundbreaking technology in proteomic research, enabling precise photolabeling and proteome extraction of subcellular organelles such as primary cilia. This study highlights Microscoop®’s ability to map the proteomic landscape of primary cilia at unprecedented resolution, identifying 4,233 proteins, including 524 known ciliary proteins critical for assembly, transportation, and signaling. By combining advanced photochemistry, microscopy, and mass spectrometry, Microscoop® opens new avenues for uncovering the intricate mechanisms of cellular structures and functions.